publications

Detection of programmed cell death using fluorescence energy transfer

Xu X, Gerard AL, Huang BC, Anderson DC, Payan DG, Luo Y.

Nucleic Acids Res. 1998 Apr 15;26(8):2034-5.

Fluorescence energy transfer (FRET) can be generated when green fluorescent protein (GFP) and blue fluorescent protein (BFP are covalently linked together by a short peptide. Cleavage of this linkage by protease completely eliminates FRET effect. Caspase-3 (CPP32) is an important cellular protease activated during programmed cell death. An 18 amino acid peptide containing CPP32 recognition sequence, DEVD, was used to link GFP and BFP together. CPP32 activation can be monitored by FRET assay during the apoptosis process.

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